This dataset comprises the phytoplankton abundance and biovolume estimates as part of a broader sampling program carried out at the Southern Ocean Time Series, a facility within the Integrated Marine Observing System (IMOS). The primary focus is sustained observing of ocean properties and processes important to climate, carbon cycling, and ocean productivity. Regular phytoplankton samples are collected at approximately 14 day intervals (exact intervals and seasonal coverage dependent on deployment length). The moorings are serviced annually, at which time the existing moorings are recovered and new moorings are deployed. Water samples from within the surface mixed-layer are collected at pre-programmed intervals using a Remote Access Sampler, in pairs about 1 hour apart. One sample in each pair is preserved for phytoplankton community composition by microscopy, and the other sample is preserved and used for accompanying chemical analysis of nutrients, total dissolved inorganic carbon (DIC) and alkalinity. Each sample is collected from within the surface mixed-layer at 32 m depth (2010 - 2017) or 5 m depth (2018-current) depending on the design of the mooring surface assembly and instrumentation. Typically a maximum of 24 samples are collected over an annual deployment cycle. The samples collected are analysed for: 1. Community composition / abundance (cells/L) 2. calculated biovolume (um3/L). Protocols for the phytoplankton time-series are described in Eriksen et al (2018), and the annual sample reports for each deployment. Data storage and access is planned to be interoperable with other national and international programs through the IMOS Infrastructure. Station metadata can be found through this resource. Data is available freely via the AODN portal: https://portal.aodn.org.au. As the taxonomic resolution of the data has changed over time, due to continual training, it is important that users refer to the change log tables included in your data download. These will provide information on the validity of the taxa, from what date we have been identifying certain taxa etc. Classification fields may be blank depending on the level to which that taxa has been identified, i.e. if only identified to family, genus and species will be blank.

This dataset comprises the zooplankton abundance estimates of a broader sampling program carried out by the National Reference Stations (NRS) within the Integrated Marine Observing system. Regular monthly zooplankton samples are undertaken at the stations as part of a larger water sampling program by the National Reference Stations. In total nine stations are sampled regularly around the Australian coastline. Sampling is conducted monthly (Esperance and Ningaloo 3 monthly) off small vessels at each of the nine reference stations. The sampling is conducted using a drop net (as per the design of Heron) which has a 60 cm diameter, 100 micron mesh and is weighted to fall at 1 m/s. The net is designed to pull closed at the end of its fall so that it samples on the way down and does not sample on the way up. The depth of the sample varies at each station. 3 zooplankton samples are taken each month. 2 are fixed in formalin and will be analysed as described below. The unfixed sample is concentrated and frozen (-80oC)and will be used for molecular analysis in the future. The Port Hacking 4 (PH4) samples (2002 - Jan 2009) were sampled using a smaller net, 20 cm diameter, which sampled on the way up and down. The PH4 site is very close to the Port Hacking national reference station (PHB) where samples are now taken to build up and enhance the time series. The plankton ecology lab (based in Queensland) will analyse the samples collected for: 1. Community composition (Taxon/m3) 2. Biomass (dry weight in mg/m3) 3. Size spectrum analysis using zooscan (in progress) Data storage and access is planned to be interoperable with other national and international programs through the IMOS Infrastructure. Station metadata can be found through this resource. Data is available freely via the AODN portal: https://portal.aodn.org.au. As the taxonomic resolution of the data has changed over time, due to continual training, it is important that users refer to the change log tables included in your data download. These will provide information on the validity of the taxa, from what date we have been identifying certain taxa etc. Classification fields may be blank depending on the level to which that taxa has been identified, i.e. if only identified to family, genus and species will be blank.

This dataset describes the logsheet information filled in during sampling at the National Reference (NRS) stations, which comprise part of a broader sampling program carried out within the Integrated Marine Observing system. Nine Sites are included in the IMOS NRS field sampling stations. Maria Island (TAS), Kangaroo Island (SA), Esperance (WA), Rottnest Island (WA), Ningaloo (WA), Darwin (NT), Yongala (QLD), North Stradbroke Island (QLD) and Port Hacking 100 (NSW). Sampling at these sites is conducted by several members of IMOS NRS Biogeochemical project. Sampling at the sites began between 29/9/2008 and 8/11/2010 and is currently ongoing for 7 sites. Sampling ceased at Ningaloo and Esperance (WA) sites in Aug/Sept 2013. Sampling is conducted monthly (Esperance and Ningaloo were 3 monthly) off small vessels. Depths sampled range from surface to 105 metres depending on the site. Depths are usually sampled at 10 metre intervals. Details including information on station locations, depths sampled and sample types collected are shown in the PDF link below extracted from the IMOS NRS NATIONAL REFERENCE STATIONS BIOGEOCHEMICAL OPERATIONS - A PRACTICAL HANDBOOK, Version 2.2 July 2012. A link to the full handbook is also provided which includes full details of the sampling regime and analyses. Historical data collected as part of CSIRO Coastal Monitoring program is available for three of the National Reference Station sites: Maria Island Coastal Station Data 1944-2008, Port Hacking 100m Coastal Station Data 1953-2010 and Rottnest Island Coastal Station Data 1951-2009. The links to the metadata and access for these data is provided below.. Data storage and access is planned to be interoperable with other national and international programs through the IMOS Infrastructure. Station metadata can be found through this resource. Data is available freely via the AODN portal: https://portal.aodn.org.au. The main goals of the project are to: 1. Provide a record of physical, chemical and biological (plankton) changes in Australian coastal waters. 2. Record data as part of a larger comprehensive national dataset, comprising both continuous in-situ sampling and analysis of discrete physical, chemical and biological samples collected monthly. People using these data should also consider historical data available from three of the National Reference Station sites: Maria Island Coastal Station Data 1944-2008 .. MarLIN Record Number: 4849 Port Hacking 100m Coastal Station Data 1953-2010 .. MarLIN Record Number: 5301 Rottnest Island Coastal Station Data 1951-2009 .. MarLIN Record Number: 4877

This dataset comprises the biomass sampling component of a broader sampling program carried out by the Plankton National Reference Station within the Integrated Marine Observing system. Regular monthly zooplankton samples are undertaken at the stations as part of a larger water sampling program by the National Reference Stations. In total nine stations are sampled regularly around the Australian coastline. Sampling is conducted monthly (Esperance and Ningaloo 3 monthly) off small vessels at each of the nine reference stations. The sampling is conducted using a drop net (as per the design of Heron) which has a 60 cm diameter, 100 micron mesh and is weighted to fall at 1 m/s. The net is designed to pull closed at the end of its fall so that it samples on the way down and does not sample on the way up. The depth of the sample varies at each station. 3 zooplankton samples are taken each month. 2 are fixed in formalin and will be analysed as described below. The unfixed sample is concentrated and frozen (-80 deg C)and will be used for molecular analysis in the future. The Port Hacking 4 (PH4) samples (2002 - Jan 2009) were sampled using a smaller net, 20 cm diameter, which sampled on the way up and down. The PH4 site is very close to the Port Hacking national reference station (PHB) where samples are now taken to build up and enhance the time series. The plankton ecology lab (based in Queensland) will analyse the samples collected for: 1. Community composition (Taxon/m3) 2. Biomass (dry weight in mg/m3) 3. Size spectrum analysis using zooscan (in progress) Data storage and access is planned to be interoperable with other national and international programs through the IMOS Infrastructure. Station metadata can be found through this resource. Data is available freely via the AODN portal: https://portal.aodn.org.au.

This dataset describes the sampling stations, which comprise part of a broader sampling program carried out by the Plankton National Reference Station within the Integrated Marine Observing system. Nine Sites are included in the IMOS NRS field sampling stations. Maria Island (TAS), Kangaroo Island (SA), Esperance (WA), Rottnest Island (WA), Ningaloo (WA), Darwin (NT), Yongala (QLD), North Stradbroke Island (QLD) and Port Hacking 100 (NSW). Sampling at these sites is conducted by several members of IMOS NRS Biogeochemical project. Sampling at the sites began between 29/9/2008 and 8/11/2010 and is currently ongoing for 7 sites. Sampling ceased at Ningaloo and Esperance (WA) sites in Aug/Sept 2013. Sampling is conducted monthly (Esperance and Ningaloo were 3 monthly) off small vessels. Depths sampled range from surface to 105 metres depending on the site. Depths are usually sampled at 10 metre intervals. Details including information on station locations, depths sampled and sample types collected are shown in the PDF link below extracted from the IMOS NRS NATIONAL REFERENCE STATIONS BIOGEOCHEMICAL OPERATIONS - A PRACTICAL HANDBOOK, Version 2.2 July 2012. A link to the full handbook is also provided which includes full details of the sampling regime and analyses. Historical data collected as part of CSIRO Coastal Monitoring program is available for three of the National Reference Station sites: Maria Island Coastal Station Data 1944-2008, Port Hacking 100m Coastal Station Data 1953-2010 and Rottnest Island Coastal Station Data 1951-2009. The links to the metadata and access for these data is provided below.. Data storage and access is planned to be interoperable with other national and international programs through the IMOS Infrastructure. Station metadata can be found through this resource. Data is available freely via the AODN portal: https://portal.aodn.org.au. The main goals of the project are to: 1. Provide a record of physical, chemical and biological (plankton) changes in Australian coastal waters. 2. Record data as part of a larger comprehensive national dataset, comprising both continuous in-situ sampling and analysis of discrete physical, chemical and biological samples collected monthly. People using these data should also consider historical data available from three of the National Reference Station sites: Maria Island Coastal Station Data 1944-2008 .. MarLIN Record Number: 4849 Port Hacking 100m Coastal Station Data 1953-2010 .. MarLIN Record Number: 5301 Rottnest Island Coastal Station Data 1951-2009 .. MarLIN Record Number: 4877

Regular monthly zooplankton samples are undertaken at the stations as part of a larger water sampling program by the National Reference Stations. In total nine stations are sampled regularly around the Australian coastline. Sampling is conducted monthly (Esperance and Ningaloo 3 monthly) off small vessels at each of the nine reference stations. The sampling is conducted using a drop net (as per the design of Heron) which has a 60 cm diameter, 100 micron mesh and is weighted to fall at 1 m/s. The net is designed to pull closed at the end of its fall so that it samples on the way down and does not sample on the way up. The depth of the sample varies at each station. 3 zooplankton samples are taken each month. 2 are fixed in formalin and will be analysed as described below. The unfixed sample is concentrated and frozen (-80oC)and will be used for molecular analysis in the future. The Port Hacking 4 (PH4) samples (2002 - Jan 2009) were sampled using a smaller net, 20 cm diameter, which sampled on the way up and down. The PH4 site is very close to the Port Hacking national reference station (PHB) where samples are now taken to build up and enhance the time series. The plankton ecology lab (based in Queensland) will analyse the samples collected for: 1. Community composition (Taxon/m3) 2. Biomass (dry weight in mg/m3) 3. Size spectrum analysis using zooscan (in progress). Data storage and access is planned to be interoperable with other national and international programs through the IMOS Infrastructure. Station metadata can be found through this resource. Data is available freely via the AODN portal: https://portal.aodn.org.au. Station metadata can be found through this resource.

This dataset comprises the phytoplankton abundance and biovolume estimates of a broader sampling program carried out by the Plankton National Reference Station within the Integrated Marine Observing system. Regular monthly phytoplankton samples are undertaken at the stations as part of a larger water sampling program by the Australian National Reference Stations. In total nine stations are sampled regularly around the Australian coastline. Sampling is conducted monthly (Esperance and Ningaloo 3 monthly) off small vessels at each of the nine reference stations. The sampling is an integrated water sample collected with niskin bottles at 10m depth intervals and preserved in lugols solution. The depth of the sample varies at each station. The plankton ecology lab (based in Queensland) will analyse the samples collected for: 1. Community composition / abundance (cells/l) 2. Biovolume (um3/L). Data storage and access is planned to be interoperable with other national and international programs through the IMOS Infrastructure. Station metadata can be found through this resource. Data is available freely via the AODN portal: https://portal.aodn.org.au. As the taxonomic resolution of the data has changed over time, due to continual training, it is important that users refer to the change log tables included in your data download. These will provide information on the validity of the taxa, from what date we have been identifying certain taxa etc. Classification fields may be blank depending on the level to which that taxa has been identified, i.e. if only identified to family, genus and species will be blank.