This record describes the Iron(II) data collected from the Marine National Facility RV Investigator voyage IN2016_V01 titled: "HEOBI: Heard Earth-Ocean-Biosphere Interactions." The voyage took place from Fremantle (WA) to Hobart (TAS) between January 7 and February 27, 2016. This dataset was produced as part of the project "Heard Earth-Ocean-Biosphere Interactions (HEOBI), GEOTRACES process study GIpr05." Samples were analysed using Flow Injection Analysis – Chemiluminescence (FIA-CL) method on-board during the voyage. Voyage, date, time, latitude, longitude, sampling site (TMR), bottle numbers and depths are included. The collected data were subsequently archived within the CSIRO Oceans and Atmosphere (O&A) Information and Data Centre (IDC) in Hobart. Additional information regarding this dataset may be contained in the Voyage Summary and the CTD Data Processing Report.