This record describes data from the ATR-FTIR and Raman spectrometers collected on the Marine National Facility RV Investigator voyage IN2015_V01. This was a trial voyage for the RV Investigator departing Hobart on the 21st March and returning to Hobart on the 29th March, 2015. The PerkinElmer Spectrum 2 Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR)Spectrometer (serial # 99683) on loan from PerkinElmer Australia. The instrument acquires infrared spectra of thin samples such as phytoplankton deposits on filters. FTIR spectra: 144 spectra from 48 samples from 6 sites Calibration of the PerkinElmer instrument performed by the company prior to the voyage. The BaySpec Agility Raman spectrometer with 1064 nm excitation laser and fibre probe optics (serial # P1480093) owned by Monash University. The instrument acquires Raman spectra of phytoplankton deposits. Raman spectra: 72 spectra from 12 samples from 2 sites Calibration of the Monash University instrument last performed 26 February 2015.