Bacterial Communities of the South Alligator River Floodplain (Kakadu) 2012
Bacterial survey of the S Alligator River Floodplain (Kakadu). The study was designed to examine how history to saltwater exposure alters microbial assemblages. The research was part of the NERP Northern Australian Hub. Data includes 90 samples taken from three different areas of energy (low, medium and high) and three areas of the floodplain (lower, upper and backwaters). The data includes processed 16S rDNA amplicons, GPS co-ordinates and soil chemistry.
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Identification info
- Alternate title
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Bacterial Communities of S Alligator River Floodplain (Kakadu)
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- Credit
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This research was funded under the NERP Northern Australia Hub, Project 5.4. The research was a collaboration between the Federal Government, CSIRO, AIMS and Charles Darwin University.
- Credit
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Contributors: Anthony Chariton, Claire Streten, Karen Gibb
- Status
- Completed
- Point of contact
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Role Organisation Electronic mail address Principal investigator CSIRO
- Spatial representation type
- Vector
- Topic category
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- Biota
- Environment
Extent
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Temporal extent
- Time position
- 2015-08-29
- Time position
- 2015-08-30
- GCMD Keywords
Resource constraints
- Use limitation
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The data have been released under the CSIRO Data Licence.
Resource constraints
- Use limitation
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All Rights (including copyright) Australian Institute of Marines Sciences, Charles Darwin University, CSIRO Australia, Department of the Enviroment 2015.
Resource constraints
- Use limitation
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The metadata and data are available to the public.
- Language
- English
- Character encoding
- UTF8
- Supplemental Information
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Project Title: 1177.2 Estuarine Health Assessment Project Description: Metabarcoding of Copper Contaminated Sediments Project Leader: Anthony Chariton Activity Title: Experiment Activity Description: Manipulative experiment using whole benthic communities obtained from an estuary in Rhode Island, USA. Activity Type: Experiment Activity Instrument: Roche 454 FLX Titanium Lead Researcher: CSIRO
Distribution Information
- Distribution format
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DIGITAL - Spreadsheets - MS Excel
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- OnLine resource
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Data Link
Data Link (DOI)
- OnLine resource
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Data Link (DOI)
Link to the data (CSIRO Data Access Portal)
Resource lineage
- Statement
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Oligonucleotide barcoded primers were used to PCR amplify a ~600 base pair (bp) product spanning the V4 and V6 hyper variable region of the 16S rRNA gene 563F, 5’-ccatctcatccctgcgtgtctccgactcag-AYTGGGYDTAAAGNG-3’ (Claesson, et al., 2010) and 1046R, 5’-cctatcccctgtgtgccttggcagtctcag-CGACAGCCATGCANCACCT- 3’ (Sogin, et al., 2006). Data were analysed using the Mothur v.1.30.1. Sequences were aligned to the SILVA database (Pruesse et al. 2007) and those that did not align were removed. Alignments were trimmed so that all sequences covered the entire alignment length. Sequences were clustered at a similarity cut-off of 97%. Representatives of resultant operational taxonomic units (OTUs) were identified using the SILVA taxonomy tool. The final dataset comprised 939,511 sequences.
- Hierarchy level
- Dataset
Reference System Information
- Reference system identifier
- EPSG/WGS 84 (EPSG:4326)
Metadata
- Metadata identifier
- c193c71c-1ff6-4860-9b70-8dc31808c285
- Language
- English
- Character encoding
- UTF8
- Contact
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Role Organisation Electronic mail address Point of contact CSIRO Oceans & Atmosphere - Hobart
Type of resource
- Resource type
- Dataset
- Date info (Creation)
- 2015-04-01T12:59:14
- Date info (Revision)
- 2015-04-01T13:51:55
Metadata standard
- Title
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ISO 19115-3:2018
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Marine Community Profile
- Date (Creation)
- 2019-05-30
- Edition
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3.0
- Edition date
- 2019-05-30T00:00:00
- Cited responsible party
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Role Organisation Electronic mail address Custodian Marine Community Profile Governance Committee
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